DL0410 Alleviates Memory Impairment in D-Galactose-Induced Aging Rats by Suppressing Neuroinflammation via the TLR4/MyD88/NF-κB Pathway

Figure 8

DL0410 inhibited the activation of the TLR4/MyD88/NF-κB signaling pathway in D-gal-stimulated rats. (a) Representative protein band images of TLR4, MyD88, p-IκBα, IκBα, p-NF-κB p65, NF-κB p65, nucleus NF-κB p65, and cytoplasm NF-κB p65 in the hippocampus. (b) Quantitative analysis of relative expression for TLR4 to β-actin in the hippocampus. (c) Quantitative analysis of relative expression for MyD88 to β-actin in the hippocampus. (d) Quantitative analysis of relative expression for p-IκB to IκB in the hippocampus. (e) Quantitative analysis of expression ratio for p-NF-κB p65 to NF-κB p65 in the hippocampus. (f) Quantitative analysis of NF-κB p65 (cytoplasm and nucleus) in the hippocampus. (g) Representative images of TLR4, MyD88, p-IκBα, IκBα, p-NF-κB p65, NF-κB p65, nucleus NF-κB p65, and cytoplasm NF-κB p65 in the cortex. (h) Quantitative analysis of relative expression for TLR4 to β-actin in the cortex. (i) Quantitative analysis of relative expression for MyD88 to β-actin in the cortex. (j) Quantitative analysis of relative expression for p-IκB to IκB in the cortex. (k) Quantitative analysis of expression ratio for p-NF-κB p65 to NF-κB p65 in the cortex. (l) Quantitative analysis of NF-κB p65 (cytoplasm and nucleus) in the cortex. Values are expressed of six independent experiments. ^#, ^##, ^### vs. the control group, , , vs. the model group.

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