Furazolidone Increases Survival of Mice Exposed to Lethal Total Body Irradiation through the Antiapoptosis and Antiautophagy Mechanism

Figure 7

FZD reduced IR-induced apoptosis and autophagy in IEC-6. (a) A dual-staining with annexin V and PI followed by the flow cytometry assay was used to examine IEC-6 cell apoptosis. Error bars are ( independent repeats), and values were calculated using two-tailed unpaired Student’s -test. (b) Flow cytometric analysis of IEC-6 cells for mtROS with MitoSOX. Error bars are ( independent repeats), and values were calculated using two-tailed unpaired Student’s -test. (c) The expression of cleaved PARP, cleaved caspase-3, BAX, and Bcl-2 by western blot in IEC-6 following different treatments. (d) The expression of MAPLC3, beclin-1, and ATG5 in IEC-6 following different treatments. (e) The expression of caspase-3 and LC3 following different treatments.

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