Dot1l Aggravates Keratitis Induced by Herpes Simplex Virus Type 1 in Mice via p38 MAPK-Mediated Oxidative Stress

Figure 2

Dot1l inhibition prevented keratitis induced by HSV-1 infection. The corneal images were taken using a slit lamp (a) and calculated for opacity score (b) at HSV-1 7dpi in mice, with or without Dot1l inhibitor EPZ004777 (10 and 50 mg/kg) treatment. H&E staining (×400) and immunofluorescence staining (×400) for CD31 were also performed (a). The mRNA levels of proinflammatory factors (c–g), including IL-1β, MMP-1, MMP-2, IL-6, and MMP-9, were detected at HSV-1 7 dpi in mice, with or without Dot1l inhibitor EPZ004777 (10 and 50 mg/kg) treatment. Data were expressed as (). versus control group; ^# versus DMSO group. Experiments were repeated 3 times.

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