Dot1l Aggravates Keratitis Induced by Herpes Simplex Virus Type 1 in Mice via p38 MAPK-Mediated Oxidative Stress

Figure 3

Dot1l inhibition prevented oxidative stress induced by HSV-1 infection. Western blot was performed for the expression of Dot1l (a) after HSV-1 infection 7 days, with or without Dot1l inhibitor EPZ004777, and quantification of their expression (b) in fold change relative to the control group. SOD activity (c), MDA content (d), and H2O2 production (e) were also detected at HSV-1 7dpi in mice, with or without Dot1l inhibitor EPZ004777. The expression of catalase, SOD1, and SOD2 were also detected by western blot and quantification of their expression (f–i) in fold change relative to the control group. Data were expressed as (). versus control group; ^# versus DMSO group. Experiments were repeated 3 times.

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