Salidroside Suppresses the Proliferation and Migration of Human Lung Cancer Cells through AMPK-Dependent NLRP3 Inflammasome Regulation

Figure 5

Effects of AMPK inhibition on ROS production and NLRP3 inflammasome activation in salidroside- (SAL-) treated A549 cells exposed to LPS. (a) In A549 cells exposed to 10 μg/ml LPS for the indicated times, cellular ROS levels were determined using DCFH-DA. (b) In A549 cells exposed to 10 μg/ml LPS in the presence of SAL at 10, 20, or 40 μM for 6 h, ROS levels were measured as above. (c) In A549 cells exposed to 10 μg/ml LPS in the presence of 40 μM SAL or 2 μM Compound C for 6 h, ROS levels were measured as described. (d) In A549 cells exposed to 10 μg/ml LPS in the presence of 40 μM SAL or 2 μM Compound C for 12 h, the levels of phosphorylated-AMPK, total-AMPK, NLRP3, caspase-1, and IL-1β in the cell lysates were determined by immunoblotting. and vs. no LPS treatment; ^# and ^## vs. treatment with LPS alone; ^& and ^&& vs. treatment with LPS plus SAL. Values are ().

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