Anti-Interleukin-16 Neutralizing Antibody Treatment Alleviates Sepsis-Induced Cardiac Injury and Dysfunction via the Nuclear Factor Erythroid-2 Related Factor 2 Pathway in Mice

Figure 4

Effects of anti-IL-16 nAb on oxidative stress in septic mice. (a) Cardiac protein expression of Nrf2, HO-1, Nox2, Nox4, and GAPDH in each group was detected and analyzed (two-way ANOVA). (b) The cardiac 4-HNE intensity of the four groups was measured (nonparametric test). (c) The activities of SOD and NADPH oxidase and the levels of GSH and MDA in serum were determined (two-way ANOVA). in each group. vs. the IgG saline group. ^# vs. the IgG LPS group. Nrf2: nuclear factor erythroid-2 related factor 2; HO-1: heme oxygenase 1; Nox2/4: nicotinamide adenine nucleotide phosphate oxidase 2/4; nAb: anti-IL-16 neutralizing antibody; LPS: lipopolysaccharide; 4-HNE: 4-hydroxynonenal; SOD: superoxide dismutase; GSH: glutathione; NADPH: nicotinamide adenine nucleotide phosphate; MDA: malondialdehyde.

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