Anti-Interleukin-16 Neutralizing Antibody Treatment Alleviates Sepsis-Induced Cardiac Injury and Dysfunction via the Nuclear Factor Erythroid-2 Related Factor 2 Pathway in Mice

Figure 7

Effects of CPUY192018 on oxidative stress and cardiomyocyte apoptosis in septic mice. (a) Nrf2, HO-1, Nox2, Nox4, and GAPDH expression in LV tissue was detected (two-way ANOVA). (b) Serum activity of SOD and NADPH oxidase and levels of GSH and MDA were measured (two-way ANOVA). (c) AIF and Cox IV expression in mitochondria and AIF, cleaved PARP, and PCNA expression in nuclei were analyzed (two-way ANOVA). in each group. vs. the nAb DMSO+LPS group. Nrf2: nuclear factor erythroid-2 related factor 2; HO-1: heme oxygenase 1; Nox2/4: nicotinamide adenine nucleotide phosphate oxidase 2/4; nAb: anti-IL-16 neutralizing antibody; CPU: CPUY192018; LPS: lipopolysaccharide; SOD: superoxide dismutase; GSH: glutathione; NADPH: nicotinamide adenine nucleotide phosphate; MDA: malondialdehyde; AIF: apoptosis-inducing factor; Cox IV: cytochrome c oxidase IV; PARP: poly-ADP-ribose polymerase; PCNA: proliferating cell nuclear antigen.

(a)

(b)

(c)