YAP-Dependent Induction of CD47-Enriched Extracellular Vesicles Inhibits Dendritic Cell Activation and Ameliorates Hepatic Ischemia-Reperfusion Injury

Figure 6

CD47⁺ EVs alleviate hepatic IRI in mouse models. (a) CD47⁺ EVs and CD47^- EVs were isolated by nonblocking anti-CD47 Ab (REA170)-FITC and anti-FITC magnetic beads. Alix, TSG101, and CD47 in CD47⁺, CD47^-, and total PMH-EVs were checked by western blot. (b) Immunogold electron microscopy images showed the immunoreactivity for CD47 on CD47⁺ and CD47^- EVs. (c) CD11c-DTR were treated with DT (4 mg/kg b.w.) and/or plus with CD47⁺ EVs (100 μg/kg b.w.) on 2 and 0 days before IRI model construction. The severity of hepatic IRI was evaluated by Suzuki’s grading [33]. (d) Hepatocellular function was assessed by serum ALT level (U/L). (e) ELISA analysis showed decreased concentrations of IL-6 and TNF-α after CD47⁺ EVs supply without CD47mAb treatment during IRI. , ; ; .

(a)

(b)

(c)

(d)

(e)