RIPK3-Mediated Necroptosis in Diabetic Cardiomyopathy Requires CaMKII Activation

Figure 5

I1PP1 overexpression reverses cardiac dysfunction, myocardial injury, and necroptosis augment in mice with DCM. 100 μL recombinant adenovirus solution carrying I1PP1 gene or vector was injected into the caudal vein of mice 12 weeks after STZ injection or injected into the caudal vein of db/db mice or its WT mice. (a) The timeline of animal treatment was summarized. (b–e) Cardiac function was assessed by echocardiography, and EF, FS, and E/A were calculated. (f) Myocardium injury was measured by HE staining.  μm. (g) cTnI was detected. (h) RIPK3 expression was quantified by western blot. GAPDH was used as a loading control. (i) Cell apoptosis of the myocardium was detected with TUNEL staining. (j) Cleaved-caspase 3 and caspase 3 protein expression were quantified by western blot. GAPDH was used as a loading control. and ; ^## and #; NS: no significance, .

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