Oxidative Medicine and Cellular Longevity

Research Article

Maternal Probiotic or Synbiotic Supplementation Modulates Jejunal and Colonic Antioxidant Capacity, Mitochondrial Function, and Microbial Abundance in Bama Mini-piglets

Table 1

Primer sequences used for intestinal mucosa antioxidant-related genes and mitochondrial-related genes.


Genes^a	Primers (5-3)	Size (bp)	Accession NO.

β-Actin	F: GATCTGGCACCACACCTTCTACAAC	107	XM_021086047.1
	R: TCATCTTCTCACGGTTGGCTTTGG
CAT	F: AGCCTACGTCCTGAGTCTCTGC	90	NM_214301.2
	R: TCCATATCCGTTCATGTGCCTGTG
GPx1	F: TGCTCATTGAGAACGTAGCGT	161	NM_214201.1
	R: CAGGATCTCCCCATTCTTGGC
GPx4	F: GATTCTGGCCTTCCCTTGC	173	NM_214407.1
	R: TCCCCTTGGGCTGGACTTT
Keap1	F: CGCCTCATCGAGTTCGCTTACAC	107	NM_001114671.1
	R: GCACGGACCACACTGTCAATCTG
CuZnSOD	F: CCAGTGCAGGTCCTCACTTCAATC	172	NM_001190422.1
	R: CGGCCAATGATGGAATGGTCTCC
MnSOD	F: GGACAAATCTGAGCCCTAACG	159	NM_214127.2
	R: CCTTGTTGAAACCGAGCC
NQO1	F: GTGGAAGCCGCAGACCTTGTG	83	NM_001159613.1
	R: CGTTCAAACCAGCCTTTCAGAATAGC
Nrf1	F: CGATGCTTCAGAATTGCCAACTACAG	125	XM_021078993.1
	R: GCGTTGTCTGGATGGTCATCTCAC
Nrf2	F: CCAATTCAGCCAGCACAACACATC	149	XM_003133500
	R: GACTGAGCCTGGTTAGGAGCAATG
ATP5A1	F: ACGCCATTGATGGAAAGGGT	98	NM_001185142.1
	R: TGGTTCCCGCACAGAGATTC
ATP5B	F: CATGTTGGGCTTTGTGGGTC	139	XM_001929410.4
	R: ATAGTCTCTGGCAGGCTGGA
PGC1α	F: ATGGAGCAATAAAGCGAAGAGCATTTG	101	NM_213963.2
	R: GAGGAGGGTCATCATTTGTGGTCAG
TFAM	F: AAATTGCTGAGCTGTGGAGGGAAC	82	NM_001130211.1
	R: TACACCTGCCAGTCTGCCCTATAAG

^aCAT: catalase; GPx1: glutathione peroxidase 1; GPx4: glutathione peroxidase 4; Keap1: kelch-like ECH-associated protein 1; CuZnSOD: copper- and zinc-containing superoxide dismutase; MnSOD: manganese-containing superoxide dismutase; NQO1: nicotinamide adenine dinucleotide (phosphate) dependency quinone oxidoreductase 1; Nrf1: nuclear factor erythroid 2-related factor 1; Nrf2: nuclear factor erythroid 2-related factor 2; mtDNA: mitochondrial DNA; ATP5A1: adenosine triphosphate synthase alpha subunit; ATP5B: adenosine triphosphate synthase β; PGC-1α: peroxisome proliferator-activated receptor γ coactivator-1α; TFAM: mitochondrial transcription factor A.