HO-1/CO Maintains Intestinal Barrier Integrity through NF-κB/MLCK Pathway in Intestinal HO-1^-/- Mice

Figure 1

CoPP and CORM-2 mice show less barrier loss and NF-κB p65/MLCK pathway activation following CCl₄ injection. C57BL/6 wildtype mice received CCl₄ injection for 12 weeks. All surviving mice were then administrated with CoPP, ZnPP, CORM-2, or iCORM-2 for the last 2 weeks. (a) Representative colon sections after hematoxylin and eosin (H&E) staining (200x, ). (b) Colon pathology scores. (c) Colonic TNF-α levels (). (d) Colon length (cm) (). (e) Representative protein bands and quantification analyses of a Western blot for (f) IκB-α, (g) p-IκB-α, (h) p-NF-κB p65/NF-κB p65, (i) long MLCK, (j) p-MLC-2/MLC-2, (k) ZO-1, and (l) occludin in the colonic epithelia. All presented data were representative of three or more independent experiments, each with similar results. , , and .

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