HO-1/CO Maintains Intestinal Barrier Integrity through NF-κB/MLCK Pathway in Intestinal HO-1^-/- Mice

Figure 3

The barrier function is dependent on HO-1 and NF-κB p65 signals from intestinal epithelial cells. (a, b) Representative protein bands and quantification of the Western blot for p-NF-κB p65/NF-κB p65, long MLCK, p-MLC-2/MLC-2, ZO-1, and occludin in Caco-2 cells transfected with FUGW-HO-1 plasmid after pretreatment of JSH-23 with or without TNF-α stimulation. (c, d) Representative protein bands of a Western blot for p-NF-κB p65/NF-κB p65, long MLCK, p-MLC-2/MLC-2, ZO-1, and occludin in Caco-2 cells transfected with pLKO.1-sh-HO-1 plasmid after pretreatment of JSH-23 with or without TNF-α stimulation. All data presented were representative of three or more independent experiments, each with similar results. and .

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