Antioxidant and Anti-Inflammatory Profiles of Spent Coffee Ground Extracts for the Treatment of Neurodegeneration

Table 3

List of primers for real-time PCR in BV-2 cells.

Gene	Primer
GAPDH forward	5ACCACAGTCCATGCCATCAC3
GAPDH reverse	5TCCACCACCCTGTTGCTGTA3
IL-1β forward	5GTTCCCATTAGACAACTGCACTACAG3
IL-1β reverse	5GTCGTTGCTTGGTTCTCCTTGTA3
TNF-α forward	5CCCCAAAGGGATGAGAAGTTC3
TNF-α reverse	5CCTCCACTTGGTGGTTTGCT3
iNOS forward	5CCTCCTCCACCCTACCAAGT3
iNOS reverse	5CACCCAAAGTGCTTCAGTCA3
COX2 forward	5TGGGGTGATGAGCAACTATT3
COX2 reverse	5AAGGAGCTCTGGGTCAAACT3