Research Article
Molecular Basis for the Interactions of Human Thioredoxins with Their Respective Reductases
Table 2
Kinetic analysis of the TrxR1 catalysed reduction of Trx1 variants in an insulin reduction-coupled assay.
| | Categories | hTrx1 mutants | | | | Catalytic efficiency | | μmol·l-1 | s-1 | % | mol·l-1·s-1 |
| | Wild-type | WT | 43 | | | 100.00% | 6.38·10-7 |
| | Inside or partly within the contact surface | K36E | 13 | | | 21.23% | 1.35·10-7 | | D60N | 16 | | | 57.23% | 3.65·10-7 | | D58,60,61N | 17 | | | 25.01% | 1.6·10-7 | | Q63R | 15 | | | 30.76% | 1.96·10-7 | | S67H | 18 | | | 118.73% | 7.57·10-7 | | K72E | 18 | | | 140.53% | 8.96·10-7 | | C73H | 16 | | | 154.25% | 9.84·10-7 |
| | Mixed type | Q63R,K94E | 15 | | | 19.46% | 1.24·10-7 |
| | Outside the contact surface but facing TrxR | K39E | 18 | | | 92.98% | 5.93·10-7 | | Q63R,K94E | 15 | | | 19.46% | 1.24·10-7 | | K94E | 6 | | | 18.66% | 1.19·10-7 |
| | Opposite side | A17R,I53R | 18 | | | 71.21% | 4.54·10-7 |
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