Novel Role of miR-18a-5p and Galanin in Rat Lung Ischemia Reperfusion-Mediated Response

Figure 3

Validation of differentially expressed miRNAs by qPCR in rat model and in vitro of IR. (a) Quantitative validation of top three upregulated and three downregulated miRNAs by qPCR from rat lung tissue with ischemia reperfusion (I/R model) or sham operation (sham) for rno-miR-540-3p, rno-miR-329-5p, rno-miR-667-5p, rno-miR-335, rno-miR-18a-5p, and rno-miR-20b-5p. (b) Quantitative validation of top three upregulated and three downregulated miRNAs by qPCR from AT2 cells with hypoxia treatment and reoxygenation (H/R model) or no treatment (normal) for rno-miR-540-3p, rno-miR-329-5p, rno-miR-667-5p, rno-miR-335, rno-miR-18a-5p, and rno-miR-20b-5p. U6 snRNA was used as an internal control. All values are normalized to those obtained from NC or WT samples (, unpaired Student’s -test, ±SEM, ).

(a)

(b)