Novel Role of miR-18a-5p and Galanin in Rat Lung Ischemia Reperfusion-Mediated Response

Figure 6

Effect of hypoxia/reoxygenation and rno-miR-18a-5p on cell proliferation and apoptosis. (a) Cell proliferation analysis from 0 h up to 72 h for AT2 cells treated with hypoxia/reoxygenation and with miR-18a-5p mimics and inhibitor. (b) Panels 2 and 3 show the profiles of AT2 cells costained with FITC-Annexin V and propidium iodide for no treatment (WT), hypoxia/reoxygenation (H/R) transfected with mimics control or with miR-18a-5p mimics. Panels 4 and 5 show the profile for cells costained with FTCI-Annexin V and propidium iodide hypoxia/reoxygenation (H/R) transfected with inhibitor control or with miR-18a-5p inhibitor. (c) Quantitative analysis of the percent apoptotic cells (early+late) from the FITC-PI profiles after treatment with hypoxia/reoxygenation, in the presence of miR18a-5p mimics and inhibitor (, unpaired Student’s -test, ±SEM).

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