Fisetin Alleviates Neointimal Hyperplasia via PPARγ/PON2 Antioxidative Pathway in SHR Rat Artery Injury Model

Figure 1

Fisetin inhibited AngII-induced proliferation in VSMCs. (a) Molecular structure of fisetin, a flavonoid compound found in plant tissues. (b) Effects of AngII on proliferation of VSMCs. A10 cells were treated with different concentrations (10^-9~10^-6 M) of AngII for 24 hours. Cell viability was determined by MTT assay. AngII with 10^-7 M was chosen to induce a VSMC proliferation model in the upcoming experiments. (c–f) Effects of fisetin on AngII-induced proliferation in VSMCs. A10 cells were treated with different concentrations (0.1~100 μM) of fisetin without or with AngII stimulation. Cell viability was determined by (c) MTT assay and cell (d) counting. Fisetin with 100 μM was selected in (e) PCNA immunofluorescence staining and (b) PCNA immunoblotting. A representative image ( μm) and the relative intensity of PCNA immunoblotting were shown ( vs. control and ^# vs. AngII-only group).

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