Mangiferin Alleviates Mitochondrial ROS in Nucleus Pulposus Cells and Protects against Intervertebral Disc Degeneration via Suppression of NF-κB Signaling Pathway

Figure 2

Mangiferin inhibited the TNF-α-induced increase in the expression of catabolic enzymes and alleviated the loss of major ECM components. HNPCs were treated with PBS, TNF-α (20 ng/ml), , or . The expression levels of (a) aggrecan, (b) Col-2, (c) MMP-13, and (d) ADAMTS-5 were measured using real-time PCR. (e–i) Levels of the aggrecan, Col-2, MMP-13, and ADAMTS-5 protein were assayed using Western blotting. (j–m) The expression levels of MMP-13 and Col-2 were detected using IF staining. Scale bar: 100 μm. All the experiments were repeated at least three times. Significant differences are indicated as follows: ns , , , , and .

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