Mangiferin Alleviates Mitochondrial ROS in Nucleus Pulposus Cells and Protects against Intervertebral Disc Degeneration via Suppression of NF-κB Signaling Pathway

Figure 5

Mangiferin alleviated the degeneration of the rat IVD in vivo. (a, b) X-ray was obtained to measure the height of the intervertebral space in rats from each group (). (c, d) MRI was used to assess the degree of IVDD in rats from each group (). (e, f) HE and SO staining were performed to analyze the IVDs in rats from each group (). Scale bar: 100 μm. (g–i) IHC was used to measure the expression of Col-2 and MMP-13 in each group (). All the experiments were repeated at least three times. Scale bar: 50 μm. Significant differences are indicated as follows: , , , and .

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