Mangiferin Alleviates Mitochondrial ROS in Nucleus Pulposus Cells and Protects against Intervertebral Disc Degeneration via Suppression of NF-κB Signaling Pathway

Table 1

Primers for real-time PCR.

Source	Target	Forward primer, 5-3	Reverse primer, 5-3
Human	COX-2	GGAACTTTCTGGTCCCTTCAG	TGTGTTTGGAGTGGGTTTCA
	iNOS	GCCAAGCTGAAATTGAATGAGGA	TTCTGTGCCGGCAGCTTTAAC
	MMP-13	TGCTGCATTCTCCTTCAGGA	ATGCATCCAGGGGTCCTGGC
	ADAMTS-5	GAACATCGACCAACTCTACTCCG	CAATGCCCACCGAACCATCT
	Collagen-II	TGGACGATCAGGCGAAACC	GCTGCGGATGCTCTCAATC
	Aggrecan	ACTCTGGGTTTTCGTGACTCT	ACACTCAGCGAGTTGTCATGG
	Bcl-2	AACATCGCCCTGTGGATGAC	AGAGTCTTCAGAGACAGCCAGGAG
	Bax	CCCGAGAGGTCTTTTTCCGAG	CCAGCCCATGATGGTTCTGAT
	c-caspase-3	GACTCTGGAATATCCCTGGACAACA	AGGTTTGCTGCATCGACATCTG
	NF-κB1	TATTTGAAACACTGGAAGCACG	CCGGAAGAAAAGCTGTAAACAT