Protective Effects and Mechanisms of Recombinant Human Glutathione Peroxidase 4 on Isoproterenol-Induced Myocardial Ischemia Injury

Figure 5

rhGPx4 reduces oxidative stress injury in H9C2 cells. (a) The viability of H9C2 cells was detected by the MTT assay. (b) H9C2 cell injury was assessed by LDH release. Data represent the (). (c) Representative images of DCFH-DA and DHE staining of H9C2 cells (fluorescence microscope, ×200). Fluorescence intensity of (d) DCF-DA and (e) DHE in H9C2 cells. (f) Representative western blot bands of Nrf2, Lamin B, HO-1, and β-actin. The expression levels of (g) Nrf2 (nucleus) and (h) HO-1 in H9C2 cells. Data represent the (). ^##, ^### vs. control; , vs. ISO.

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