Protective Effects and Mechanisms of Recombinant Human Glutathione Peroxidase 4 on Isoproterenol-Induced Myocardial Ischemia Injury

Figure 8

rhGPx4 inhibits apoptosis in H9C2 cells. (a) Representative images of Annexin-V/PI staining of H9C2 cells. (b) The apoptotic rates of H9C2 cells. The apoptosis rate is the sum of the early and late apoptosis rates. (c) Representative western blot bands of Bcl-2, Bax, Cleaved caspase-3, and β-actin. (d) The ratio of the Bcl-2/Bax protein expression in H9C2 cells. (e) The expression levels of cleaved caspase-3 in H9C2 cells. Data represent the (). ^##, ^### vs. control; , vs. ISO.

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