HIF-1 Inhibitor YC-1 Reverses the Acquired Resistance of EGFR-Mutant HCC827 Cell Line with MET Amplification to Gefitinib

<div>Colony formation analysis in HCC827 GR cells with different treatments. HCC827 GR cells were seeded and cultured on dishes with different treatments (blank control, YC-1, gefitinib, YC-1, and gefitinib combined, for 16 h and 28 h), then, cells were culture for 2 weeks in media without drugs. (a) Colony formation of HCC827 GR cells observed by naked eyes. (b) Colony formation of HCC827 GR cells observed under microscope (magnification, ×40). (c) Quantified results of colony formation analysis. The concentration of YC-1 was 40 <i>μ</i>M, and the final concentration of gefitinib was 20 nM. 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Oxidative Medicine and Cellular Longevity

fig4

Figure 4

Figure 4: HIF-1 Inhibitor YC-1 Reverses the Acquired Resistance of EGFR-Mutant HCC827 Cell Line with MET Amplification to Gefitinib 