HIF-1 Inhibitor YC-1 Reverses the Acquired Resistance of EGFR-Mutant HCC827 Cell Line with MET Amplification to Gefitinib

Figure 8

Expression of p-EGFR, EGFR, HIF-1α, p-Met, and c-Met in HCC827 GR cells with different treatments. (a) Western blot analysis was performed to detect p-EGFR, EGFR, HIF-1α, p-Met, and c-Met protein expression levels in HCC827 GR cells with different treatments (blank control, YC-1, gefitinib, YC-1, and gefitinib combined, for 16 h and 28 h). (b) Quantified densitometric scanning of the blots. The concentration of YC-1 was 40 μM, and the final concentration of gefitinib was 20 nM. Error bars represented the . Data were obtained from three independent experiments. .

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