RIPK3 Induces Cardiomyocyte Necroptosis via Inhibition of AMPK-Parkin-Mitophagy in Cardiac Remodelling after Myocardial Infarction

Figure 1

RIPK3 upregulation promoted cardiac remodelling after MI (). (a, b) The change of RIPK3 expression after MI. The infarct size (c, d) and the degree of fibrosis (e, f) of mice hearts were performed by Masson’s trichrome staining. Ripk3 genetic ablation reduced both the heart weight-to-body weight ratio (g) and the lung weight-to-body weight ratio (h). (i, j) The mRNA expression levels of myocardial atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) were performed by RT-PCR. (k) The change of plasma BNP levels was detected by ELISA. (l, m) Loss of Ripk3 reduced the mRNA levels of proinflammatory cytokines TNF-α and IL-6 in the peri-infarcted areas of the heart tissue. (n) ROS content in the heart tissue was detected by DHE staining. (o–q) The change of protein levels of phosphoglycerate mutase 5 (PGAM5) and phosphorylated mixed-lineage kinase domain-like protein (p-MLKL). Data are shown as the , .

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