Dexmedetomidine Alleviates Hypoxia-Induced Synaptic Loss and Cognitive Impairment via Inhibition of Microglial NOX2 Activation in the Hippocampus of Neonatal Rats

Figure 7

Overexpression of NOX2 in BV2 microglia eliminated the protective effect of dexmedetomidine against hypoxia-induced neuronal damage. (a) The infection efficiency of the control lentivirus (vector) and NOX2 overexpression lentivirus were measured by Western blot analysis. NOX2 expression is presented as the percentage of that in the Control group. Primary hippocampal neurons were treated with microglia-CM for 24 h; thereafter, (b) cell viability was detected by CCK-8 assay, and (c, d) neuronal apoptosis was measured by Annexin V/PI staining and flow cytometry. Representative images (c) and graphical quantification (d) of apoptosis are presented. The data are expressed as the , . vs. the Control group, ^# vs. the Hypoxia group, ^& vs. the Hypo+Pre-Dex+Vector or Hypo+Post-Dex+Vector group. ns: no significance; Hypo: hypoxia; Pre-Dex: dexmedetomidine pretreatment; Post-Dex: dexmedetomidine posttreatment.

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