Effects of Lipid Peroxidation-Mediated Ferroptosis on Severe Acute Pancreatitis-Induced Intestinal Barrier Injury and Bacterial Translocation

Figure 3

The expression of ferroptosis core proteins ACSL4, GPX4, and FTH1 in ileum was detected by Western blotting (a–d). Quantitative real-time PCR analysis of ACSL4 and IREB2 mRNA transcription (e, f). Using GAPDH as an internal control to standardize the data, the results showed folding change compared with the SO group. The ultrastructure of IECs was observed by TEM at 24 h (g). White arrows indicate shrunken mitochondria. All data are expressed as the (). The variables between groups were compared by Student’s -test and one-way ANOVA. and vs. the SO group; and vs. the SAP group.

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