Plasma Small Extracellular Vesicle-Carried miRNA-501-5p Promotes Vascular Smooth Muscle Cell Phenotypic Modulation-Mediated In-Stent Restenosis

Figure 4

The effect of miRNA-501-5p on smooth muscle cell proliferation and migration. (a) Representative blots and quantified data showing the expression of vascular smooth muscle cell- (VSMC-) specific contractile marker (α-SMA and calponin) in primary human aortic smooth muscle cells (HASMC) transfected with miRNA-501-5p mimic. (b) Representative confocal images showing immunofluorescent staining for VSMC-specific contractile marker (α-SMA, green) to determine VSMC identity. Nuclei were stained with DAPI (blue). The VSMC proliferation was evaluated with Edu-positive cells (c) and MTT assay (d) in HASMC transfected with miRNA-501-5p mimic. The VSMC migration was measured using Transwell (e) and wound healing assay (f) in HASMC transfected with miRNA-501-5p mimic. Effects of miRNA-501-5p inhibitor on MTT assay (g), immunofluorescent staining of α-SMA (h), Edu assay (i), and transwell assay (j), in HASMC treated with plasma sEV, then transfected with miRNA-501-5p inhibitor or not. per group in (d) and (g); per group in other groups. NC: negative control. Values are . , , and .

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