Plasma Small Extracellular Vesicle-Carried miRNA-501-5p Promotes Vascular Smooth Muscle Cell Phenotypic Modulation-Mediated In-Stent Restenosis

Figure 7

The potential origin of miRNA-501-5p and the proposed schematic. qRT-PCR analysis of miRNA-501-5p (a) and pre-miRNA-501-5p (b) in tissues from rats with carotid artery balloon injury (CABI) or not. Data are normalized to U6 (). (c) qRT-PCR analysis showing that the expressions of miRNA-501-5p and pre-miRNA-501-5p are significantly higher in rat aorta with endothelium (endo+) than that without endothelium (endo-). Data are normalized to U6 (). (d) Drug-eluting stent implantation increases the production of miRNA-501-5p in multiple tissues, especially in endothelial cells. miRNA-501-5p, cargoed in sEV, is delivered to the vascular smooth muscle cells (VSMC) by plasma sEV and promotes VSMC proliferation and migration by targeting Smad3. Values are . , , and .

(a)

(b)

(c)

(d)