Long Noncoding RNA ANPODRT Overexpression Protects Nucleus Pulposus Cells from Oxidative Stress and Apoptosis by Activating Keap1-Nrf2 Signaling

Figure 3

ANPODRT knockdown aggravates TBHP-induced human NP cell injury. Human NP cells were transfected with short interfering RNA (siRNA) against ANPODRT (si-ANPODRT) or negative control (si-NC) and then exposed to TBHP. (a) The expression of ANPODRT in human NP cells was detected by qRT-PCR. (b) Cell viability of human NP cells was detected by CCK-8 assay. (c) Intracellular ROS production and (d) MDA level in the human NP cells. (e) Annexin V-APC/7-AAD staining results showing the rate of apoptosis in human NP cells. (f–h) The protein levels of cytoplasmic cytochrome c (cyto-cyt c) and cleaved caspase-3 (c-caspase-3) in the human NP cells were measured by Western blotting. Data are represented as the . and versus control group; ^#, ^##, and ^### versus TBHP+si-NC group.

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