Acetylshikonin Induces Apoptosis in Human Colorectal Cancer HCT-15 and LoVo Cells via Nuclear Translocation of FOXO3 and ROS Level Elevation

Figure 2

FACS analysis of annexin V/PI double-stained HCT-15 and LoVo cells. HCT-15 and LoVo cells were treated with 0, 1.25, 2.5, and 5 μM of acetylshikonin for 24 and 48 h. (a, b) The treated cells were then stained with annexin V and PI for apoptotic analysis. The percentages of apoptotic cells are indicated on the plots. (c, d) Percentages of each portion in apoptotic cells were represented in bar graphs. The data represent the of three independent experiments. Single and double asterisks indicate significant differences from the control cells ( and , respectively).

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