Acetylshikonin Induces Apoptosis in Human Colorectal Cancer HCT-15 and LoVo Cells via Nuclear Translocation of FOXO3 and ROS Level Elevation

Figure 5

ROS generation in colorectal cancer HCT-15 and LoVo cells treated with acetylshikonin. (a, c) Intracellular ROS generation in acetylshikonin-treated HCT-15 and LoVo cells and those cotreated with NAC was measured by using DCFH-DA (10 μM) and flow cytometry after 6 h of treatment. Mean fluorescence intensity (MFI) at each concentration is indicated on each plot. Mean fluorescence intensity (MFI) indicated on each plot. (b, d) Bar graph shows quantitation of the MFI. The vector control MFI was set at 100%. The data represent the of three independent experiments. Single and double asterisks indicate significant differences from the control cells ( and , respectively). The data represent the of three independent experiments. Number sign indicates a significant difference of NAC-treated cells from acetylshikonin-treated cells ().

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