Histone Methyltransferase G9a Promotes the Development of Renal Cancer through Epigenetic Silencing of Tumor Suppressor Gene SPINK5

Figure 2

Inhibition of G9a methylation inhibits proliferation with simultaneous induction of apoptosis. (a) 786-O and SN12C cells were treated with different concentrations of UNC0638 (0, 0.5, 1, 2, 4, 8, 16, and 32 μM) for 24 h, 48 h, and 72 h. CCK-8 assay was performed to measure cell growth level. (b–e) 786-O and SN12C were treated with different concentrations of UNC0638 for 24 h and Annexin-V-FITC/PI double-staining assay to detect cell apoptosis through flow cytometry. (f, g) Colony formation experiments were performed to detect how different concentrations of UNC0638 (treated for 24 h) affected proliferation of 786-O and SN12C. (H) The expression of protein-related apoptosis pathway and H3K9me2 was measured by western blot (treated for 24 h).

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