Histone Methyltransferase G9a Promotes the Development of Renal Cancer through Epigenetic Silencing of Tumor Suppressor Gene SPINK5

Figure 4

Loss of G9a causes a decrease in the carcinogenicity of RCC cell lines. (a) A stable G9a knockdown RCC line in 786-O was constructed using a lentivirus and expression of G9a, and H3K9me2 was measured by western blot. (b) CCK-8 assay was performed to show the proliferation ability of G9a knockdown RCC line. (c) Detection of apoptosis rate of shG9a-786-O cells via Annexin V-PE/7-AAD double staining (treated for 24 h; WT: wild type; NC: negative control). (d) Colony formation experiments were performed to detect cell proliferation after knocking down G9a. (e) Invasive abilities of shG9a-786-O cells were measured through transwell assay. (f) Migratory abilities of shG9a-786-O cells were measured through wound healing assay (treated for 24 h). (g, h) The expression of protein-related EMT and apoptosis pathway was measured through western blot.

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