Histone Methyltransferase G9a Promotes the Development of Renal Cancer through Epigenetic Silencing of Tumor Suppressor Gene SPINK5

Figure 6

G9a can affect the proliferation and EMT process of RCC cells in vivo. (a) Xenograft tumors in each group were harvested and extracted completely after 30 days. (b) Each time point represents mean tumor volume for each group. (c) Tumor weight was obtained at the end of experiment. (d) Immunohistochemical staining was performed to demonstrate that the EMT index of G9a knockdown in vitro showed significant changes. (e) The expression of protein-related EMT and apoptosis pathway and G9a was measured by western blot. (f) The expression of proteins P53 and SPINK5 was measured by immunohistochemical staining in tumors.

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