Research Article
Neferine Protects against Hypoxic-Ischemic Brain Damage in Neonatal Rats by Suppressing NLRP3-Mediated Inflammasome Activation
Figure 5
Neferine effectively reduces CoCl2-incuded PC12 cell injury. (a, b) PC12 cells were treated for 12 h and 24 h in a CoCl2 dose-dependent manner as assessed with CCK8. (c, d) PC12 cells were treated with different concentrations of neferine for 12 h and 24 h simultaneously under 1000 mΜ CoCl2 treatment, and cell viability was determined by CCK8. (e, f) The levels of each mRNAs in PC12 were normalized to those of β-actin for each group. , , and versus the sham group. , , and versus the HI group. . (g) Representative images of TUNEL staining (green). The nucleus (blue) was labeled with DAPI in PC12. Scale . (h) Quantification of TUNEL staining in PC12. . .
(a) |
(b) |
(c) |
(d) |
(e) |
(f) |
(g) |
(h) |