Increase in Blood-Brain Barrier (BBB) Permeability Is Regulated by MMP3 via the ERK Signaling Pathway

Figure 3

MMP3 enhances the anesthetic effect of isoflurane. (a) The genotypes of WT (WT+/+), heterozygous MMP3-KO (MMP3–/+), and homozygous MMP3-KO (MMP3–/–) mice were confirmed via PCR; the WT and KO alleles were identified with 517-bp and 670-bp fragments, respectively. (b) MMP3 protein abundance was evaluated via ELISA in the serum of WT and homozygous MMP3-KO (KO) mice. . (c–e) WT mice, MMP3-KO mice, and WT mice treated with MMP3 were anesthetized with inhaled isoflurane, and the (c) time to the onset of anesthesia, (d) time to emergence from anesthesia, and (e) total volume of isoflurane was recorded. .

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