Increase in Blood-Brain Barrier (BBB) Permeability Is Regulated by MMP3 via the ERK Signaling Pathway

Figure 5

MMP3 reduces the integrity of BMVEC monolayers. (a, c) TEER measurements were recorded in monolayers of WT and MMP3-KO (KO) BMVECs during treatment with the indicated combinations of phosphate-buffered saline (PBS), isoflurane, MMP3, and/or NNGH; treatment was administered one hour after TEER was initiated. (b, d) Endothelial barrier integrity was evaluated by calculating the mean TEER. , ^####. (e, f) WT or MMP3-KO (KO) BMVECs were grown with primary mouse astrocytes in Transwell chambers and treated with PBS, LPS, LPS, and NNGH, or MMP3, as indicated; then, the chambers were suspended in the wells of a 6-well plate, (e) red (40KD) or (f) blue (3KD) FITC-dextran was added to the chamber, and permeability was evaluated 24 hours later by measuring the intensity of dextran fluorescence in the plate wells. , , , ^####. Quantified data are summarized for 5 independent experiments.

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