Uterine Insulin Sensitivity Defects Induced Embryo Implantation Loss Associated with Mitochondrial Dysfunction-Triggered Oxidative Stress

Figure 5

High-fat-induced-uterine insulin resistance beginning before pregnancy impaired endocrine status and embryo implantation in mice. Female mice of 8-week-age were fed with a CON diet or HFD (D12492 experimental diet) for 16 weeks and then mated with normal male mice. Day 1 of pregnancy was considered as the day when vaginal plug was observed. Uterine tissues and serum were collected at indicated time. (a) Representative blot and quantification of protein abundance of IRS1, p-IRS1^Ser307, p85α, p110α, Akt, p-Akt^Ser473, AS160, and p-AS160^Thr462 in the uterus on day 5 of pregnancy. Levels of estradiol (b) and progesterone (c) and serum lipid changes (d) were determined on day 5 of pregnancy. Uterine receptivity-related gene changes on days 4 (e) and 5 (f) of pregnancy and the number of implantation site on days 6 (g), 11 (h), and 18 (i) of pregnancy were also recorded. Data are values ( for (b–f), for (g)). Student’s test was used for statistical analysis. and . CON: control; HFD: high-fat diet.

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