Effect of Verapamil, an L-Type Calcium Channel Inhibitor, on Caveolin-3 Expression in Septic Mouse Hearts

Figure 4

Western blot analysis of CAV3. (a) The amounts of CAV3 in the sham () and SH+VP () groups were measured 24 hours after sham operation. The amounts of CAV3 in the SSI () and SSI+VP () groups were measured 6, 12, and 24 hours after CLP surgery and expressed in arbitrary units (AUs). GAPDH was used to determine equivalent loading conditions. Note that the expression of CAV3 was significantly increased in the SSI group 24 hours after CLP as compared to SSI+VP and sham group mice. Statistical analysis was performed by one-way ANOVA followed by Tukey’s test. Data were expressed as the ; (SSI vs. SSI+VP) and (SSI vs. sham). (b) The autoradiograph resulting from western blot analysis of representative protein levels for CAV3 and GAPDH of mouse hearts, subjected to sham operation (sham, SH+VP) or sepsis induction (SSI, SSI+VP) 24 hours after surgery.

(a)

(b)