Manoalide Shows Mutual Interaction between Cellular and Mitochondrial Reactive Species with Apoptosis in Oral Cancer Cells

Figure 1

Detection of several kinds of reactive species and cell viability in oral cancer cells after 6 h manoalide treatment. Cells were treated under control (0.1% DMSO only) and manoalide (10 μM) treatments for 6 h. All experiments had the same concentration of DMSO. (a) Fluorescence staining images of cellular and mitochondrial radical probes with DCFH-DA, HPF, DAF-FM, DHE, and MitoSOX Red in manoalide-treated oral cancer (Ca9-22 and CAL 27) cells. For each treatment, the light microscope, radical probe, and Hoechst 33342 (2-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5-bi-1H-benzimidazole trihydrochloride trihydrate) counterstaining images were provided. DCFH-DA-, HPF-, and DAF-FM-probed images were captured by Leica DMi8 fluorescence microscope. DHE- and MitoSOX-probed images were captured by Olympus FV1000 confocal microscope. (b) MTS assay for cell viability determination. Results between control and manoalide treatment of the same cells are considered significantly different (indicated via different letters without overlapping) (). Data, ( independent experiments, each experiment was performed with three replications).

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