Manoalide Shows Mutual Interaction between Cellular and Mitochondrial Reactive Species with Apoptosis in Oral Cancer Cells

Figure 2

Flow cytometry of radical probes with DCFH-DA, HPF, DAF-FM, DHE, and MitoSOX Red in manoalide-treated oral cancer cells. Cells were treated with control (0.1% DMSO only) and manoalide (10 μM) for 0, 10 min, 1 h, and 6 h. All experiments had the same concentration of DMSO. (a) Flow cytometry patterns for manoalide-treated oral cancer cells (Ca9-22 and CAL 27). (b) Statistics. The reactive mean intensity for the control is set to 1. Results from different treatments are considered significantly different for multiple comparison (indicated via different letters without overlapping) ( to 0.0001). In the example of (b), the DCFH-DA fold for control, 10 min, 1 h, and 6 h show letters at top for “f,” “de,” “c,” and “a” for Ca9-22 cells. Since they were marked with different letters without overlapping, all the treatments between each other for control, 10 min, 1 h, and 6 h differ significantly. Moreover, the DCFH-DA fold at 6 h for Ca9-22 and CAL 27 cells show letters at top for “a” and “b,” indicating that they are significantly different. Data, ( independent experiments, each experiment collected with 10000 gated cell counts).

(a)

(b)