Manoalide Shows Mutual Interaction between Cellular and Mitochondrial Reactive Species with Apoptosis in Oral Cancer Cells

Figure 3

Apoptosis effects of inhibitors for cellular reactive species, mitochondrial reactive species, and apoptosis (NAC, MT, and ZVAD) in manoalide-treated oral cancer cells. Cells (Ca9-22 and CAL 27) were pretreated with control (0.1% DMSO only), NAC (8 mM), MT (20 μM), and ZVAD (100 μM) for 2 h and posttreated with control and manoalide (10 μM) for 0 and 6 h. All experiments had the same concentration of DMSO. (a) Flow cytometry patterns of annexin V/7AAD staining for manoalide-treated oral cancer cells. (b) Statistics of (a). (c) Flow cytometry patterns of pancaspase activity for manoalide-treated oral cancer cells. (d) Statistics of (c). Results from different inhibitor treatments are considered significantly different compared to control (indicated via different letters without overlapping) ( to 0.0001). Data, ( independent experiments, each experiment collected with 10000 gated cell counts).

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