Melatonin Alleviates Contrast-Induced Acute Kidney Injury by Activation of Sirt3

Figure 1

Melatonin administration ameliorated renal function and reduced the expression of NGAL, renal tubular injure score after iohexol-treatment in vivo. (a) The ratio of KW to BW in different groups. (b, c) The level of SCr and BUN in different groups. (d) Representative immunohistochemical staining of NGAL. (e) Quantification of NGAL immunostaining. (f) Representative micrographs of HE-stained of kidney on morphological changes. (g–j) Semiquantitative analysis of interstitial edema, cytoplasmic vacuolar changes, intratubular cast formation, and luminal congestion to evaluated renal tubular injures. KW: kidney weight. BW: body weight. SCr: serum creatinine. BUN: blood urea nitrogen. NGAL: neutrophil gelatinase-associated lipid. HE: hematoxylin and eosin staining. All experiments were calculated at least 3 times. Data are presented as the means±SEM. , , , .

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