Ferroportin-Dependent Iron Homeostasis Protects against Oxidative Stress-Induced Nucleus Pulposus Cell Ferroptosis and Ameliorates Intervertebral Disc Degeneration In Vivo

Figure 1

TBHP treatment induced cell viability decline, ROS accumulation, and ferroptosis in human NPCs. (a, b, e, f) The human NPCs were treated with TBHP (25–100 μM) or DMSO for 6 h or 12 h. The quantitative values are expressed as (); versus DMSO (6 h), ^# versus DMSO (12 h). (a) Cell proliferation was detected using EdU staining under fluorescence microscope and the positive cells were quantitated. (b) Cell viability was examined by the absorbance of CCK-8. (c, d, g, h) The human NPCs were treated with DMSO, TBHP (50 μM), Fer-1 (5 μM), Lip-1 (5 μM), or DFO (100 μM) for 12 h. The quantitative values are expressed as (); , TBHP versus DMSO; , TBHP+ Fer-1/Lip-1/DFO versus TBHP. (c) Cell proliferation was detected using EdU staining under fluorescence microscope, and the positive cells were quantitated. (d) Cell viability was examined by the absorbance of CCK-8. (e–h) Lipid ROS levels were assayed using C11-BODIPY 581/591 by flow cytometry. (i) Observation of human NPC morphologic change after treatment with TBHP by TEM. The quantification of mitochondrial size is expressed as (); TBHP versus DMSO.

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