Resveratrol Alleviates 27-Hydroxycholesterol-Induced Senescence in Nerve Cells and Affects Zebrafish Locomotor Behavior via Activation of SIRT1-Mediated STAT3 Signaling

Figure 2

The 27HC induces STAT3 activation via SIRT1. (a) BV2 and PC12 cells were treated with 10 μM 27HC and 10 nM E2. Western blot analysis was performed for SIRT1, Ac-STAT3Lys685, and β-Actin expression. CON group: ethanol treatment. (b) The quantitative analysis of protein bands of (a). , statistically significant difference from the CON group. SIRT1: (BV2: , 27HC group; , E₂ group) (PC12: , 27HC group; , E2 group); AC-STAT3^Lys685: (BV2: , 27HC group; , E₂ group) (PC12: , 27HC group; , E₂ group). Data are expressed as the of three independent experiments. (c) BV2 and PC12 cells were treated with si-NC or siRNA-SIRT1 for 12 h. Western blot analysis was performed for SIRT1, p-STAT3^Tyr705, Ac-STAT3^Lys685, and β-actin expression. The two sets are experimental replicates. (d) The quantitative analysis of protein bands of (c). , statistically significant difference from the si-NC group. BV2: , SIRT1; , p-STAT3^Tyr705; , Ac-STAT3^Lsy685; PC12: , SIRT1; , p-STAT3^Tyr705; , Ac-STAT3^Lsy685. (e) qRT-PCR analysis of IL-6 and SIRT1. , statistically significant difference from the si-NC group. (BV2: , SIRT1; , IL-6; PC12: , SIRT1; , IL-6). Data are expressed as the of three independent experiments.

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