Resveratrol Alleviates 27-Hydroxycholesterol-Induced Senescence in Nerve Cells and Affects Zebrafish Locomotor Behavior via Activation of SIRT1-Mediated STAT3 Signaling

Figure 4

REV inhibits 27HC-induced senescence and STAT3 activation. BV2 and PC12 cells were treated with 10 μM 27HC, , or 20 μM REV alone for 12 h. CON group: ethanol treatment. (a) Cells were subjected to an SA-β-Gal staining assay. Scale bars: 50 μm. (b) The aged cells were stained in blue, and the number of stained cells from panels was counted. , statistically significant difference from the CON group. BV2: ; PC12: . ^#, statistically significant difference from the 27HC-treated group. BV2: ; PC12: . Data are expressed as the of three independent experiments. BV2 and PC12 cells were treated with 10 μM 27HC or for 12 h. CON group: ethanol treatment. (c) Western blot analysis of SIRT1, Ac-STAT3^Lys685, p-STAT3^Tyr705, and β-actin. (d) The quantitative analysis of protein bands of (c). , statistically significant difference from the CON group. BV2: (SIRT1: ; Ac-STAT3^Lys685: ; p-STAT3^Tyr705: ); PC12: (SIRT1: ; Ac-STAT3^Lys685: ; p-STAT3^Tyr705: ). ^#, statistically significant difference from the 27HC-treated group. BV2: (SIRT1: ; Ac-STAT3^Lys685: ; p-STAT3^Tyr705: ); PC12: (SIRT1: ; Ac-STAT3^Lys685: ; p-STAT3^Tyr705: ). (e) qRT-PCR analysis of IL-6. , statistically significant difference from the CON group. BV2: ; PC12: . ^#, statistically significant difference from the 27HC-treated group. BV2: ; PC12: . Data are expressed as the of three independent experiments.

(a)

(b)

(c)

(d)

(e)