Resveratrol Alleviates 27-Hydroxycholesterol-Induced Senescence in Nerve Cells and Affects Zebrafish Locomotor Behavior via Activation of SIRT1-Mediated STAT3 Signaling

Figure 5

Effects of 27HC on ROS generation in zebrafish larvae. (a) Effect of different concentrations of 27HC on zebrafish embryo mortality (). CON group: ethanol treatment. , statistically significant difference from the CON group. , 10 μM 27HC group; , 20 μM 27HC group; , 30 μM 27HC group; , 40 μM 27HC group. (b) Staining for ROS in larvae at 4 dpf following 36 hpf to 4 dpf treatment with 20 μM 27HC, , or 10 nM E2. CON group: ethanol treatment. Scale bars: 50 μm. (c) The levels of ROS fluorescence were determined using flow cytometric analysis (). REV attenuated 27HC-induced aging in zebrafish larvae. , statistically significant difference from the CON group (). ^#, statistically significant difference from the 27HC-treated group (). (d) SA-β-Gal staining in zebrafish embryos at 4 dpf following 36 hpf to 4 dpf treatment with 20 μM 27HC, 10 nM E2, , or . CON group: ethanol treatment. (e) Quantitative analysis of SA-β-Gal staining in larvae (). , statistically significant difference from the CON group (). ^#, statistically significant difference from the 27HC-treated group. , 27HC+NAC group; , 27HC+REV group. Data are expressed as mean ± SD of three independent experiments.

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