Chinese Herbal Prescription Fu-Zheng-Qu-Xie Prevents Recurrence and Metastasis of Postoperative Early-Stage Lung Adenocarcinoma: A Prospective Cohort Study Followed with Potential Mechanism Exploration

Figure 4

The FZQX prescription suppressed the LLC/A549 proliferation, migration, and invasion by regulating MDSCs. (a, b) The frequencies of MDSCs in different drug concentration gradients were determined through flow cytometry analysis. (c) MDSC activation-related mRNA expression levels were determined by RT-qPCR. (d) LLC/A549 cell proliferation was detected by colony formation assay. (e) LLC/A549 cell invasion was detected by Transwell assay. (f) LLC/A549 cell migration was examined by wound healing assay. (g) Quantification of the colony formation assay, the Transwell assay, and the wound healing assay results determined by ImageJ software. Control: LLC/A549 cell with no intervention; MDSC-CM: LLC/A549 treated with conditional medium (CM) derived from MDSCs culture; FZQX-CM: LLC/A549 treated with CM derived from the FZQX prescription culture; FZQX: LLC/A549 treated with the FZQX prescription.

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