Chinese Herbal Prescription Fu-Zheng-Qu-Xie Prevents Recurrence and Metastasis of Postoperative Early-Stage Lung Adenocarcinoma: A Prospective Cohort Study Followed with Potential Mechanism Exploration

Figure 5

The FZQX prescription suppressed the activation IL-1β/NF-κB signaling pathway in MDSCs. (a) Culture supernatants were collected and analyzed for cytokine concentration by ELISA. (b, c) The mRNA expression levels of IL-1β/NF-κB signaling pathway-related genes were determined by RT-qPCR, including TRAF6, cFLIP, XIAP, and CCL4. (d) Western blot detected the expression levels of key proteins related to tumor proliferation, invasion, and metastasis involved in the IL-1β/NF-κB signaling pathway, including VEGFR, MMP9, PCNA, and involved in signaling pathways, including Bcl2, IL-1β, NF-κB, and STAT3. (e) Immunofluorescence detected the expression levels of Ki-67, MMP9, pNF-κB, and IL-1β. Control (MDSCs-CM): MDSCs conditional medium; FZQX: MDSCs-CM intervened by the FZQX prescription; IL-1β: MDSCs-CM intervened by IL-1β; IL-1RA: MDSCs-CM intervened by IL-1 receptor antagonist; IL-1β+FZQX: MDSCs-CM intervened by IL-1β and the FZQX prescription simultaneously; IL-1RA+FZQX: MDSCs-CM intervened by IL-1RA and the FZQX prescription simultaneously. IL-1β+ IL-1RA: MDSCs-CM intervened by IL-1β and IL-1RA.

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